Figure 7.

Inhibition of Ebola infection by raft-disrupting agents filipin and nystatin. Vero E6 cells were left untreated or treated for 30 min with 0.2 μg/ml of filipin or 100 U/ml of nystatin at 37°C, washed extensively with PBS, and infected with EBOV at an MOI of 1. As a control for lack of general toxicity and persistent effect on viral replication, upon treatment with Filipin, cells were washed, and incubated in medium for 4 h before infection with EBOV (Filipin→recovery). After 48 h supernatants were harvested and viral titers determined by plaque assay.