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. 2003 May 19;197(10):1279–1289. doi: 10.1084/jem.20030044

Figure 5.

Figure 5.

The UGT2B17 gene is deleted in donor cells and HLA-A*2902 positive cells from unrelated individuals that are not recognized by PL8 CTL. (A) UGT2B17 consists of six exons encoded over 27 Kb of DNA on chromosome 4. Primer pairs used for PCR to detect exon 1 and exon 6 sequences were selected such that at least one primer of each pair contained nucleotides that were mismatched with all other known UGT family members. The primer pair for the region immediately 5′ to the UGT2B17 start site was selected to amplify nt −370 to −30. (B) SSP-PCR for exon 1 and exon 6 sequences of UGT2B17 and PCR for 5′ sequences upstream of UGT2B17 on genomic DNA prepared from B-LCL. PCR products for exon 1, exon 6 and the 5′ region upstream of UGT2B17 were detected in the recipient B-LCL and unrelated HLA-A29+ B-LCL that were recognized by PL8 CTL. These PCR products were sequenced and found to be identical to UGT2B17 (unpublished data). No PCR products were detected in donor B-LCL or unrelated HLA-A29+ B-LCL that were not lysed by PL8 CTL. The B-LCL from unrelated donors (indicated as 1–4) are the same lines used for analysis of gene expression and cytotoxicity in Fig. 4, A and B. PCR for GAPDH was performed as a control.