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. 2003 Oct;2(5):930–936. doi: 10.1128/EC.2.5.930-936.2003

FIG. 3.

FIG. 3.

A 32-kDa band disappears in ahp1Δ cells. Strains SY3358 (wild-type), SY3806 (urm1Δ), SY3805 (uba4Δ), and SY3815 (ahp1Δ) were grown to mid-log phase in YEPD at 30°C. The lysates were analyzed by SDS-PAGE and immunoblot analysis using affinity-purified polyclonal anti-Urm1p antibodies and monoclonal antibodies to Dpm1p (to confirm equal protein loading). The L bracket designates high-molecular-weight Urm1p-modified species, while the asterisk designates the position of a 32-kDa Urm1p-modified species.