TABLE 1.
Yeast strains used in this study
| Straina | Relevant genotypeb | Source or reference |
|---|---|---|
| SY3357 | MATaleu2-Δ1 ura3-52 his3-Δ200 trpl-Δ63 ade8Δade2-101 mfa2-Δ1::FUS1-lacZ | 39 |
| SY3358 | MATα leu2-Δ1 ura3-52 lys2-801 his3-Δ200 trpl-Δ63 ade8Δade2-101 mfa2-Δ1::FUS1lacZ | 39 |
| SY3362 | SY3357, except cla4Δ::TRP1 (pRS316ADE8CLA4) | 39 |
| SY3754 | SY3358, except cla4Δ::TRP1 ste20Δ::TRP1 (pRS316ADE8CLA4) | 16 |
| SY3805 | SY3358, except uba4Δ::KanMX6 | 16 |
| SY3806 | SY3358, except urmlΔ::KanMX6 | 16 |
| SY4117 | SY3357, except urmIΔ::HIS3 (PGALI pRS315 URMI) | This study |
| SY4139 | SY3357, except urmIΔ::HIS3 | This study |
| SY4118 | SY3357, except urmIΔ::HIS3 (PGALI pRS315 HFURMI) | This study |
| Y258 | pep4-3 his4-580 ura3-52 leu2-3, 112 | 55 |
| SY3972 | pep4-3 his4-580 ura3-52 leu2-3,112 (pEGH PGALI-GST-HisX6-AHPI) | 55 |
| SY4050 | pep4-3 his4-580 ura3-52 leu2-3, 112 (pEGH PGALI-GST-HisX6) | 55 |
| BY4741 | MATahis3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Research Genetics |
| SY3839 | BY4741 urm1Δ::KanMX4 | Research Genetics |
| SY3840 | BY4741 uba4Δ::KanMX4 | Research Genetics |
| SY4119 | BY4741 ahp1Δ::KanMX4 | Research Genetics |
| SY3807 | SY3357, except cla4Δ::TRP1 uba4Δ::HIS3 (pRS316ADE8CLA4) | 16 |
| SY3808 | SY3357, except cla4Δ::TRP1 urm1Δ::KanMX6 (pRS316ADE8CLA4) | 16 |
| SY4120 | SY3357, except cla4Δ::TRP1 ahp1Δ::KanMX6 (pRS316ADE8CLA4) | This study |
| HYL333 | MATα ura3-52 | G. Fink |
| SY3826 | HYL333, except his3Δ ura3 | 16 |
| SY3827 | SY3826, except uba4Δ::KanMX6 | 16 |
| SY3828 | SY3826, except urm1Δ::KanMX6 | 16 |
| SY4121 | SY3826, except ahp1Δ::KanMX6 | This study |
| SY3836 | BY4741, except gln3Δ::KanMX4 | Research Genetics |
| SY4122 | BY4741, except urm1Δ::KanMX4 ahp1Δ::NatMX4 | This study |
| SY4123 | BY4741, except uba4Δ::KanMX4 ahp1Δ::NatMX4 | This study |
| SY4124 | BY4741, except urm1Δ::KanMX4 uba4Δ::KanMX4 | This study |
a
SY3357 and SY3358 are derivatives of S288C.
b
Strains containing the designation ura3 were made URA3 at the designated chromosomal locus and then ura3 by selection on 5-fluoroorotic acid as described in Materials and Methods.