Figure 2.

In vitro colony-forming activity of purified stem and progenitors by ectopic hGM-CSF signaling. Clonogenic analysis of stem and progenitor cells purified from hGM-CSFR transgenic mice. Single cells were sorted into 96-well plates and cultured in methylcellulose for 8 d in the presence of cytokine cocktail (Slf, IL-3, IL-11, mGM-CSF, Epo, and Tpo), hGM-CSF alone, or mGM-CSF alone. 288 single cells were analyzed in each assay. Note that hGM-CSF alone, like the cytokine cocktail, can support formation of all myeloid lineage colonies.