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. 2003 May 19;197(10):1311–1322. doi: 10.1084/jem.20021843

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Copyright © 2003, The Rockefeller University Press

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Figure 5. — hGM-CSF signals can induce lineage switch from the lymphoid to the myeloid pathways in vitro. (A) FACS^® analysis of 200 purified hGM-CSFR⁺ CLPs and pro-T cells cultured in the presence of hGM-CSF alone. Note that both CLPs and pro-T cells give rise to Gr-1⁺ Mac-1⁺ granulocytes/monocytes (GM) and Mac-1^hi CD11c⁺ I-Ab⁺ myeloid dendritic cells (DC). (B) Clonogenic analysis of myeloid differentiation of CLPs and pro-T cells by ectopic hGM-CSF signals. Single cells were cultured in a Terasaki plate in the presence of cyto-kine cocktail (Slf, IL-3, IL-11, mGM-CSF, Epo, Tpo, and hGM-CSF), hGM-CSF alone, or mGM-CSF alone. hGM-CSFR⁺ CLPs and pro-T cells exhibit a significant differentiation potential into myeloid lineage cells including granulocytes, monocytes, and dendritic cells at high frequencies. 240 single cells were analyzed in each assay. (C) Morphology of a single pro-T cell derived GM colony (top) and a myeloid DC colony (bottom). Giemsa staining: ×1,000. (D) FACS^® analysis of progeny of 40 hGM-CSFR⁺ CLPs on an S17 stromal layer in the presence of 10 ng/ml IL-7 for 10 d. 20 ng/ml hGM-CSF was added to the culture at the indicated time points. Myeloid progeny became undetectable if hGM-CSF was added >2 d after the initiation of culture.

Figure 5. — hGM-CSF signals can induce lineage switch from the lymphoid to the myeloid pathways in vitro. (A) FACS^® analysis of 200 purified hGM-CSFR⁺ CLPs and pro-T cells cultured in the presence of hGM-CSF alone. Note that both CLPs and pro-T cells give rise to Gr-1⁺ Mac-1⁺ granulocytes/monocytes (GM) and Mac-1^hi CD11c⁺ I-Ab⁺ myeloid dendritic cells (DC). (B) Clonogenic analysis of myeloid differentiation of CLPs and pro-T cells by ectopic hGM-CSF signals. Single cells were cultured in a Terasaki plate in the presence of cyto-kine cocktail (Slf, IL-3, IL-11, mGM-CSF, Epo, Tpo, and hGM-CSF), hGM-CSF alone, or mGM-CSF alone. hGM-CSFR⁺ CLPs and pro-T cells exhibit a significant differentiation potential into myeloid lineage cells including granulocytes, monocytes, and dendritic cells at high frequencies. 240 single cells were analyzed in each assay. (C) Morphology of a single pro-T cell derived GM colony (top) and a myeloid DC colony (bottom). Giemsa staining: ×1,000. (D) FACS^® analysis of progeny of 40 hGM-CSFR⁺ CLPs on an S17 stromal layer in the presence of 10 ng/ml IL-7 for 10 d. 20 ng/ml hGM-CSF was added to the culture at the indicated time points. Myeloid progeny became undetectable if hGM-CSF was added >2 d after the initiation of culture.