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. 2003 May 19;197(10):1323–1334. doi: 10.1084/jem.20021952

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Copyright © 2003, The Rockefeller University Press

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Figure 6. — Requirement of Bax and/or Bak for NFX*- or CPX-induced cell death. (A) SV40-transformed WT MEF or Bax^−/− Bak^−/− DKO cells were exposed to NFX* and CPX for the indicated period, followed by determination of the frequency of DiOC₆(3)^low and annexin V–positive cells. (B) MEF lacking Bax, Bak, Bax and Bak (DKO), or Bid were exposed to either NFX* or CPX and the indicated parameters were measured. (C) NFX*- and CPX-induced cathepsin B release in control and Bax^−/− Bak^−/− DKO cells, as determined by immunostaining. The percentage of cells exhibiting diffuse cathepsin B staining as well as those with diffuse cytochrome c staining has been quantified. (D) Failure of chemical cathepsin inhibition to prevent NFX*- or CPX-induced cell death. HeLa cells were pretreated with the indicated cathepsin inhibitors, followed by determination of the frequency of DiOC₆(3)^low and PI^high cells. (E) Cathepsins are dispensable for NFX*- or CPX-induced cell death. MEF with the indicated genotypes were treated with NFX* or CPX, and apoptotic parameters were measured as in D. Results are means ± SD of three independent determinations.