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. 2003 May 19;197(10):1323–1334. doi: 10.1084/jem.20021952

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Copyright © 2003, The Rockefeller University Press

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Figure 8. — Differential involvement of ROS in CPX- and NFX-induced cell death. (A) Relationship between ROS production and ΔΨ_m loss triggered by NFX* and CPX. HeLa cells were exposed to NFX* or CPX for the indicated interval, and stained simultaneously with the ΔΨ_m-sensitive dye DiOC₆(3) and the ROS-sensitive probe hydroethidine (HE), whose oxidation by superoxide anions yields the red fluorescent product ethidium (Eth). Note that NFX*treatment causes ROS production before the ΔΨ_m drops, whereas after CPX treatment, ROS production is only observed in a subpopulation of DiOC₆(3)^low cells. (B–E) Effects of antioxidants on CPX- and NFX-induced cell death. HeLa cells were pretreated (60 min) with the cell-permeable GSH ethyl ester (10 mM) or the superoxide dismutase analogue MnTBAP (100 μM), followed by overnight treatment with NFX* or CPX and determination of the frequency of , ROS-overproducing (B), PS-exposing, dead (C) cells, and cells exhibiting Bax in the apoptotic conformation (D, determined with the conformation-specific 6A7 mAB as in Fig. 5 A) and diffuse cathepsin B staining (E, determined as in Fig. 1 D).

Inline graphic — Differential involvement of ROS in CPX- and NFX-induced cell death. (A) Relationship between ROS production and ΔΨ_m loss triggered by NFX* and CPX. HeLa cells were exposed to NFX* or CPX for the indicated interval, and stained simultaneously with the ΔΨ_m-sensitive dye DiOC₆(3) and the ROS-sensitive probe hydroethidine (HE), whose oxidation by superoxide anions yields the red fluorescent product ethidium (Eth). Note that NFX*treatment causes ROS production before the ΔΨ_m drops, whereas after CPX treatment, ROS production is only observed in a subpopulation of DiOC₆(3)^low cells. (B–E) Effects of antioxidants on CPX- and NFX-induced cell death. HeLa cells were pretreated (60 min) with the cell-permeable GSH ethyl ester (10 mM) or the superoxide dismutase analogue MnTBAP (100 μM), followed by overnight treatment with NFX* or CPX and determination of the frequency of , ROS-overproducing (B), PS-exposing, dead (C) cells, and cells exhibiting Bax in the apoptotic conformation (D, determined with the conformation-specific 6A7 mAB as in Fig. 5 A) and diffuse cathepsin B staining (E, determined as in Fig. 1 D).