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. 2003 May 19;197(10):1323–1334. doi: 10.1084/jem.20021952

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Copyright © 2003, The Rockefeller University Press

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Figure 9. — Effect of the Bax/Bak DKO on apoptosis induced by two different lysomal stimuli. (A) Chloroquine-induced ΔΨ_m loss and cell death is inhibited by Baf A₁ as well as by ablation of Bax and Bak. WT MEF or DKO MEF were exposed to 30 μg/ml chloroquine (24 h), followed by determination of DiOC₆(3)/PI staining and flow cytometric analysis. (B) Chloroquine-induced cathepsin B translocation, cytochrome c release, and chromatin condensation are inhibited in DKO cells. Cells, treated as in A, were subjected to immunofluorescence analysis (anti–cathepsin B, anti–cytochrome c, and Hoechst 33324), and the cells exhibiting the indicated phenotype were determined. (C) DAP kinase-induced cell death and ΔΨ_m loss in WT and DKO MEF. Cells were transfected with the constitutively active DAP kinase construct ΔCaM or controls. After 72 h, the cells were stained with DiOC₆(3) and DAPI, and the percentage of (DiOC₆(3)^low) or dead (DAPI⁺) cells was measured by cytofluorometry.

Inline graphic — Effect of the Bax/Bak DKO on apoptosis induced by two different lysomal stimuli. (A) Chloroquine-induced ΔΨ_m loss and cell death is inhibited by Baf A₁ as well as by ablation of Bax and Bak. WT MEF or DKO MEF were exposed to 30 μg/ml chloroquine (24 h), followed by determination of DiOC₆(3)/PI staining and flow cytometric analysis. (B) Chloroquine-induced cathepsin B translocation, cytochrome c release, and chromatin condensation are inhibited in DKO cells. Cells, treated as in A, were subjected to immunofluorescence analysis (anti–cathepsin B, anti–cytochrome c, and Hoechst 33324), and the cells exhibiting the indicated phenotype were determined. (C) DAP kinase-induced cell death and ΔΨ_m loss in WT and DKO MEF. Cells were transfected with the constitutively active DAP kinase construct ΔCaM or controls. After 72 h, the cells were stained with DiOC₆(3) and DAPI, and the percentage of (DiOC₆(3)^low) or dead (DAPI⁺) cells was measured by cytofluorometry.