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Uptake of Tat fusion proteins by human PMNs, FACS® analysis. Flow cytometric analysis of the uptake of Tat fusion proteins by neutrophils. The cells were incubated with labeled Tat-AP (A and D) or Tat-CT (B, C, and E), either with 1 μM Tat construct for the indicated time at 37°C (A, B, and C), or for 60 min at 37°C with the indicated concentration of Tat construct (D and E). In C, cells were also preincubated with 100 μM chloroquine for 30 min at 37°C.
