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Inhibition of TNF-triggered neutrophil spreading by Tat-CT. Neutrophils were plated on FBS-coated glass coverslips and preincubated or not with 500 nM Tat-CT at 37°C for 30 min before stimulation with 100 ng/ml TNF, 100 ng/ml PMA, or an equal volume of KRPG (Cont.), and fixed and photographed with phase-contrast microscope. ×1,000.
