Figure 7.

Inhibition of TNF-induced phosphorylation of endogenous Pyk2 by Tat-CT. Neutrophils were preincubated with Tat-CT or ovalbumin (ova; each 500 nM) at 37°C for 30 min and stimulated with TNF (T) or buffer alone as a control (C). Total cell lysates were separated by SDS-PAGE and western-blotted (WB) with (A) antiphosphotyrosine Ab or (B) Abs specific for individual phosphotyrosine (PY) residues of Pyk2. One membrane was stripped and reprobed with anti-Pyk2 Ab, demonstrating equal loading of the lanes.