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. 2003 Jan 6;197(1):63–75. doi: 10.1084/jem.20021638

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Copyright © 2003, The Rockefeller University Press

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Figure 8. — TNF-induced association of Pyk2 and Syk, and the inhibition of tyrosine phosphorylation of Syk by wortmannin and Tat-CT. (A) Pyk2 was immunoprecipitated (IP) from neutrophils stimulated with TNF (T) or treated with buffer alone as a control (C) and Western blotted (WB) for Syk. (B) Syk was immunoprecipitated from neutrophils after preincubation with or without TNF (T) and/or 10 nM wortmannin and Western blotted for phosphotyrosine (PY; top) or Syk (bottom). (C) Syk was immunoprecipitated from neutrophils after preincubation with or without TNF (T) and/or with Tat-CT or ovalbumin (ova; each 500 nM) at 37°C for 30 min and probed with antiphosphotyrosine Ab (PY) or anti-Syk.