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. 2003 Jan 6;197(1):7–17. doi: 10.1084/jem.20021229

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Copyright © 2003, The Rockefeller University Press

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Figure 5. — Mycobacteria induce IL-10 production by DCs through ManLAM and direct infection. (a) ManLAM induces IL-10 production of LPS-matured DCs. Immature DCs were incubated with 15 μg/ml of either ManLAM or AraLAM in the presence of LPS (10 ng/ml). The specificity was determined in the presence of blocking antibodies against DC-SIGN (AZN-D2; 20 μg/ml). Supernatants were harvested after 18 h and the IL-10 production was measured by ELISA. Values are the means ± standard deviations of triplicate determinations. One representative experiment out of three is shown. (b) M. bovis BCG infection of immature DCs induces IL-10 production. Immature DCs were infected with M. bovis BCG (MOI 4), and the experiment was performed as described for panel a. Values are the means ± standard deviations of triplicate determinations. One representative experiment out of three is shown.