Figure 6.

ManLAM inhibits LPS-induced DC activation through DC-SIGN binding. (a) ManLAM does not induce activation of immature DCs. Immature DCs were incubated with ManLAM, AraLAM, or LPS for 18 h, and activation was determined by measuring the expression of CD80, CD86, CD83, and HLA-DR. Dotted lines represent isotype controls, the thin lines indicate expression levels of immature DCs, and the thick line represents immature DCs that have been treated with either LPS (10 ng/ml), ManLAM (15 μg/ml), or AraLAM (15 μg/ml). One representative experiment out of three is shown. (b) LPS-induced activation of DCs is blocked by ManLAM. Immature DCs were cocultured with LPS alone, or together with either ManLAM or AraLAM for 18 h. Dotted lines represent isotype controls. Thick lines, and the mean fluorescence values in the histograms, represent the expression levels after treatment with LPS alone, or in combination with either ManLAM or AraLAM. Thin lines indicate the presence of antibodies against DC-SIGN throughout the incubation. One representative experiment out of three is shown.