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. 2003 Jan 6;197(1):7–17. doi: 10.1084/jem.20021229

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Copyright © 2003, The Rockefeller University Press

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Figure 7. — M. bovis BCG induces maturation and ManLAM inhibits the induced DC activation through DC-SIGN binding. (a) M. bovis BCG induces maturation of immature DCs. Immature DCs were incubated with LPS or viable M. bovis BCG (MOI 4, 20, and 100) for 18 h, and activation was determined by measuring the expression of CD80, CD86, CD83, and HLA-DR. The mean fluorescence intensity (MFI) of the difference between specific antibody and isotype control is depicted. One representative experiment out of three is shown. (b) M. bovis BCG-induced activation of DCs is blocked by ManLAM. Immature DCs were infected with M. bovis BCG (MOI 4). Cells were preincubated with 15 μg/ml of either ManLAM or AraLAM and the expression of the markers was measured after 18 h as described for panel a. Specificity was determined by preincubating cells with blocking antibodies against DC-SIGN (AZN-D2; 20 μg/ml). One representative experiment out of three is shown.