Table I.
Composition of Mucosal Lymphoid Populations in H. hepaticus–infected RAG−/− Mice
| Tissue | Group | Cell no. | Neuts | Monos | DC | NK | CD4+
T |
|---|---|---|---|---|---|---|---|
| % | % | % | % | % | |||
| LPL | Control | 2.8 × 104 | 0.6 | 18.2 | 8.8 | 3.0 | 0.3 |
| Hh | 2.1 × 105 | 17.5 | 13.0 | 6.0 | 8.1 | 0.3 | |
| Hh + 25+ | 2.5 × 104 | 0.4 | 14.6 | 7.3 | 5.8 | 15.2 | |
| MLN | Control | 1.4 × 105 | 1.8 | 15.1 | 4.5 | 47.7 | 0.2 |
| Hh | 3.6 × 105 | 4.0 | 31.7 | 4.7 | 25.2 | 0.3 | |
| Hh + 25+ | 7.7 × 105 | 0.5 | 10.2 | 5.6 | 15.8 | 22.1 |
Groups of 129SvEvRAG2−/− mice were left untreated (Control), or infected by oral gavage with 2 × 108 cfu H. hepaticus (Hh), or infected with H. hepaticus and reconstituted with 1 × 105 CD4+ CD45RBlowCD25+ T cells (Hh + 25+). Mice were sacrificed 8–12 wk later and LPL and MLN populations (pooled from 4–6 mice per group) were characterized using FACS® analyses. Mean cell number for each tissue was calculated by dividing the total number of cells isolated by the number of mice pooled. Results shown are representative of two similar experiments.