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. 2003 Jan 6;197(1):51–62. doi: 10.1084/jem.20020617

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Copyright © 2003, The Rockefeller University Press

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Figure 5. — Enhanced expression of CD86 and APC function by activated DR6^−/− B cells. (A) B220⁺ cells from DR6^−/− or WT mice were stimulated with 20 μg/ml anti-IgM, 10 μg/ml anti-CD40, or 5 μg/ml LPS for 24 h and analyzed by flow cytometry for CD86 surface expression. Solid line histograms represent CD86 staining of activated cells and dashed line histograms represent unstimulated levels. (B) As in A but LPS-stimulated B cell cultures were analyzed for surface expression of MHC class II I-A^b (top) and CD80 (bottom). Y axis represents relative cell number and x axis represents log₁₀ fluorescence intensity. (C) For allogeneic T cell proliferation assay, responding T cells (H-2^d) were purified from BALB/c mice and cultured in the presence or absence of irradiated WT or DR6-deficient (−/−) B cells that were previously stimulated with 10 μg/ml anti-CD40 for 24 h and then irradiated. After 5 d of incubation, the proliferation of allogeneic T cells was measured by [³H]thymidine incorporation in the last 6 h of pulse. Values shown represent the mean and error bars represent the SD. *, P < 0.01. Data shown are representative of three independent experiments.

Figure 5. — Enhanced expression of CD86 and APC function by activated DR6^−/− B cells. (A) B220⁺ cells from DR6^−/− or WT mice were stimulated with 20 μg/ml anti-IgM, 10 μg/ml anti-CD40, or 5 μg/ml LPS for 24 h and analyzed by flow cytometry for CD86 surface expression. Solid line histograms represent CD86 staining of activated cells and dashed line histograms represent unstimulated levels. (B) As in A but LPS-stimulated B cell cultures were analyzed for surface expression of MHC class II I-A^b (top) and CD80 (bottom). Y axis represents relative cell number and x axis represents log₁₀ fluorescence intensity. (C) For allogeneic T cell proliferation assay, responding T cells (H-2^d) were purified from BALB/c mice and cultured in the presence or absence of irradiated WT or DR6-deficient (−/−) B cells that were previously stimulated with 10 μg/ml anti-CD40 for 24 h and then irradiated. After 5 d of incubation, the proliferation of allogeneic T cells was measured by [³H]thymidine incorporation in the last 6 h of pulse. Values shown represent the mean and error bars represent the SD. *, P < 0.01. Data shown are representative of three independent experiments.