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. 2003 Jan 6;197(1):101–109. doi: 10.1084/jem.20021908

Figure 2.

Figure 2.

Myeloid and plasmacytoid bone marrow–derived DCs induce Th1 development with CpG whereas only myeloid DCs are responsive to LPS. FACS®-purified bone marrow–derived DCs were cultured with 1 μM OVA and CD4+ Mel14+ T cells for 9 d under neutral conditions with LPS or CpG. To some cultures, anti–IL-12p40 mAbs were added. At day 9, the percentage of IL-4 and IFN-γ+ T cells was determined by flow cytometry after restimulation with PMA and ionomycin.