Table I.
Predicted and Observed Recombination Efficiencies for Physiologic and Synthetic RS
| Test vector | RIC | R (%) | Assoc. gene segment |
RS |
|---|---|---|---|---|
| p290T-2310 | −22.5 | 3.5 ± 1.2 | VH7S3*01 | GATCT CACAGTG AGGGTACTTCAGTGTGAGCCTAG ACACAAACC AGATCC |
| p290Tspa3 | −27.7 | 6.1 ± 1.6 | Synthetic | GATCT CACAGTG ACGGAGATAAAGGAGGAAGCAGG ACAAAAACC AGATCC |
| p290Ta | −28.8 | 7.6 ± 2.1 | Jk1*1 | GATCT CACAGTG GTAGTACTCCACTGTCTGGCTGT ACAAAAACC AGATCC |
| p290Thep3 | −28.7 | 3.7 ± 2.4 | Synthetic | GATCT CACAGTA GTAGTACTCCACTGTCTGGCTGT ACAAAAACC AGATCC |
| p290Thepγ | −30.2 | 5.5 ± 1.4 | Synthetic | GATCT CACACTG GTAGTACTCCACTGTCTGGCTGT ACAAAAACC AGATCC |
| p290T-2304 | −31.1 | 2.4 ± 1.1 | Vα7-4*01 | GATCT CACAGTG CTCTCCAGGCACCTGCGGGCTGC ACCCAAACC AGATCC |
| p290Tnon3 | −31.4 | 5.6 ± 2.8 | Synthetic | GATCT CACAGTG GTAGTACTCCACTGTCTGGCTGT ACAGAAACT AGATCC |
| p290T-2305 | −32.7 | <0.4 | VH7S4*02 | GATCT CACAGTG AGGGTACTTCAGTGTGAGCCTAG AAAAAAACC AGATCC |
| p290Thepnon3 | −32.8 | 2.9 ± 1.5 | Synthetic | GATCT CACAGTA GTAGTACTCCACTGTCTGGCTGT ACAGAAACT AGATCC |
| p290Tnonγ | −33.8 | 1.9 ± 1.3 | Synthetic | GATCT CACAGTG GTAGTACTCCACTGTCTGGCTGT ACTGAAAAT AGATCC |
| p290Thepnonγ | −35.7 | 1.0 ± 0.4 | Synthetic | GATCT CACACTG GTAGTACTCCACTGTCTGGCTGT ACTGAAAAT AGATCC |
| p290T-2301 | −35.8 | 0.6 ± 0.6 | Vβ13-2*1 | GATCT CACAGTG ATGTGGGGTTTCCTCCCCTCTGC ACAGAAAGG AGATCC |
| p290T3 | −36.8 | 2.5 ± 1.3 | Vλ3 | GATCT CACAGTA ACGGAGATAAAGGAGGAAGCAGG ACAGAAACT AGATCC |
| p290T-2306 | −40.0 | 2.4 ± 0.8 | Vδ1*01 | GATCT CACAGTG GTCTACAGTCAGCCACAGGCTGT CTCCAAACC AGATCC |
| p290T-2302 | −40.1 | <0.4 | JH1*1 | GATCT CACAGTC TCTGTTCTGCCTCTGTTCCTATA CTAAAACTC AGATCC |
| p290T-2303 | −40.6 | 1.2 ± 0.5 | Vα4-4/Vδ10*01 | GATCT CACAGTG CTCCCAGCAAGGCTGGAGCCTGG CCCCAAACT AGATCC |
| p290Tspaγ | −49.8 | 0.9 ± 0.6 | Synthetic | GATCT CACAGTG AAGGACCTGGAATAGGCAAGAAA ACAAAAACC AGATCC |
| p290TMDLCγ | −57.1 | <0.3 | Putative cRS | GATCT CACACTG AAGGACCTGGAATAGGCAAGAAA ACTGAAAAT AGATCC |
| pJH290 12-RSb | Synthetic | TCGAC CACAGTG CTACAGACTGGA ACAAAAACC CTGCAG | ||
| p290-1201 | −8.0 | 30.9 ± 8.9 | Vκ4-86 | TCGAC CACAGTG ATACAGACTGGA ACAAAAACC CTGCAG |
| p290-1202 | −12.4 | 36.7 ± 3.8 | Vκ4-79 | TCGAC CACAGTG AAACAGACTAGA ACAAAAACC CTGCAG |
| p290-1204 | −13.7 | 11.4 ± 4.1 | Vκ10-94 | TCGAC CACAATG ATATAAGTCATA ACATAAACC CTGCAG |
| p290-1203 | −14.2 | 18.0 ± 5.9 | Vκ8-27 | TCGAC CACAATG CTTCAGCCTCCT ACAAAAACC CTGCAG |
| p290-1205 | −18.7 | 3.3 ± 1.0 | Vκ17-121 | TCGAC CACAGTG CTATGTCCTCTT ACAGAAACC CTGCAG |
| p290-1206 | −24.7 | 15.9 ± 5.0 | Jα34 | TCGAC CACAGTG ATATCATGTTCT ACAAAAACC CTGCAG |
| p290-1207 | −28.4 | 16.3 ± 2.8 | Jα12 | TCGAC CACAGTG TTTCTTAGTCAG TCAAAAACA CTGCAG |
| p290-1208 | −32.3 | 6.6 ± 1.4 | Jα4 | TCGAC CACAGTA GAAAGGTGCTTT ACAAGAATT CTGCAG |
| p290-1209 | −37.8 | 1.1 ± 0.7 | Jβ2-2 | TCGAC CACAGTC GTCGAAATGCTG GCACAAACC CTGCAG |
| p290-1210 | −48.2 | <0.3 | Jb1-2 | TCGAC CACAGTC GTCGAAATGCTG GCACAAACC CTGCAG |
Recombination efficiency of physiologic and synthetic RS. The recombination efficiency, R, is measured by PCR as described in Materials and Methods. The 12- and 23-RS are listed with coding- and signal-flanks in italics, heptamer and nonamer in bold, and the spacer in plain text.
a
12-RS variants were tested for rearrangement to this 23-RS.
b
23-RS variants were tested for rearrangement to this 12-RS. This RS is synthetic; its R and RIC are therefore not included in Fig. 4.