FIG. 5.

Control of NRG1 expression by doxycycline affects the outcome of haematogenously disseminated candidiasis caused by strain SSY50-B. Groups of mice either on 5% sucrose alone (NO DOX) or on sucrose containing doxycycline (DOX) were injected, as indicated, with different doses of the modified strain SSY50-B grown overnight in the absence of doxycycline, and their survival was monitored over a period of 28 days. While mice on doxycycline succumbed to the infection at rates similar to those seen with wild-type strains, every mouse not exposed to the antibiotic survived even at the highest dose tested (5 × 10⁶ CFU). Statistically significant differences were observed between each two groups of mice injected with the same yeast inoculum in the presence or absence of doxycycline in their drinking water (P < 0.01 for all comparisons).