Table II.
Analysis of Coding and RS Joining in ArtN/N ES Cells
| Cell lines | (Ampr + Camr)/Ampr | Percent | Relative level |
|---|---|---|---|
| Experiment 1a | |||
| TC1 | 9,868/2,042,500 | 0.48 | 1.0 |
| ArtN/N (82.10.66) | 387/862,000 | 0.04 | 0.08 |
| ArtN/N (82.10.77) | 114/493,500 | 0.02 | 0.04 |
| XRCC4−/− | 6/52,133 | 0.01 | 0.02 |
| Experiment 2 | |||
| TC1 | 6,414/1,435,000 | 0.45 | 1.0 |
| ArtN/N (82.10.77) | 188/547,000 | 0.03 | 0.07 |
| DNA-PKcsN/N | 11/191,500 | 0.006 | 0.01 |
| XRCC4−/− | 21/246,500 | 0.009 | 0.02 |
| Experiment 3 | |||
| TC1 | 2,625/500,000 | 0.53 | 1.0 |
| ArtN/N (82.10.66) | 270/1,345,000 | 0.02 | 0.04 |
| ArtN/N (82.10.77) | 164/1,410,000 | 0.01 | 0.02 |
| DNA-PKcsN/N | 62/1,100,000 | 0.006 | 0.01 |
| Ku70−/− | 25/300,000 | 0.008 | 0.02 |
| Experiment 4 | |||
| TC1 | 33/3,199 | 1.0 | 1.0 |
| ArtN/N (82.10.66.2) | 50/126,000 | 0.05 | 0.05 |
| DNA-PKcsN/N | 12/90,000 | 0.01 | 0.01 |
| Experiment 5 | |||
| TC1 | 1,503/503,250 | 0.30 | 1.0 |
| ArtN/N (82.10.66.2) | 136/346,500 | 0.04 | 0.13 |
| DNA-PKcsN/N | 66/378,500 | 0.02 | 0.07 |
| Experiment 6 | |||
| TC1 | 2,499/844,500 | 0.30 | 1.0 |
| ArtN/N (82.10.66) | 80/107,933 | 0.07 | 0.2 |
| ArtN/N (82.10.77) | 10/74,133 | 0.01 | 0.04 |
| XRCC4−/− | 0/36,467 | <0.003 | <0.01 |
| Experiment 7 | |||
| TC1 | 188/17,667 | 1.1 | 1.0 |
| ArtN/N (82.10.66.2) | 36/19,000 | 0.19 | 0.17 |
| ArtN/N (82.10.77) | 48/59,867 | 0.08 | 0.07 |
| DNA-PKcsN/N (2-41-3) | 9/61,000 | 0.01 | 0.009 |
| DNA-PKcsN/N (2-35-2) | 4/54,400 | 0.007 | 0.006 |
| TC1 (no RAG) | 23/70,467 | 0.03 | 0.03 |
| + hArt cDNA | |||
| ArtN/N (82.10.66.2) | 248/17,333 | 1.4 | 1.3 |
| ArtN/N (82.10.77) | 410/69,133 | 0.59 | 0.55 |
| + mDNA-PKcs cDNA | |||
| DNA-PKcsN/N (2-41-3) | 181/27,667 | 0.65 | 0.59 |
| DNA-PKcsN/N (2-35-2) | 336/76,900 | 0.44 | 0.40 |
| Experiment 8 | |||
| TC1 | 3,850/640,000 | 0.6 | 1.0 |
| ArtN/N (82.10.66.2) | 107/147,000 | 0.07 | 0.12 |
| ArtN/N (82.10.77) | 70/292,000 | 0.02 | 0.04 |
| DNA-PKcsN/N (2-41-3) | 17/78,000 | 0.02 | 0.04 |
| DNA-PKcsN/N (2-35-2) | 12/65,300 | 0.02 | 0.04 |
| TC1 (no RAG) | 10/146,000 | 0.007 | 0.01 |
| + hArt cDNA | |||
| ArtN/N (82.10.66.2) | 118/6,940 | 1.7 | 2.8 |
| ArtN/N (82.10.77) | 1,037/148,000 | 0.7 | 1.2 |
| + mDNA-PKcs cDNA | |||
| DNA-PKcsN/N (2-41-3) | 2,389/115,800 | 2.1 | 3.4 |
| DNA-PKcsN/N (2-35-2) | 1,755/378,900 | 4.6 | 7.6 |
| Experiment 1b | |||
| TC1 | 552/107,266 | 0.51 | 1.0 |
| ArtN/N (82.10.66) | 450/57,533 | 0.78 | 1.5 |
| ArtN/N (82.10.77) | 551/70,400 | 0.78 | 1.5 |
| XRCC4−/− | 0/12,467 | <0.008 | <0.02 |
| Experiment 2 | |||
| TC1 | 5,643/852,500 | 0.66 | 1.0 |
| ArtN/N (82.10.77) | 4,319/394,500 | 1.09 | 1.7 |
| DNA-PKcsN/N | 526/141,000 | 0.37 | 0.6 |
| XRCC4−/− | 6/184,000 | 0.003 | 0.005 |
| Experiment 3 | |||
| TC1 | 2,011/414,000 | 0.49 | 1.0 |
| ArtN/N (82.10.77) | 3,540/615,000 | 0.58 | 1.2 |
| ArtN/N (82.10.66) | 5,240/765,000 | 0.68 | 1.4 |
| DNA-PKcsN/N | 1,016/659,500 | 0.15 | 0.3 |
| Ku70−/− | 25/473,500 | 0.005 | 0.01 |
| Experiment 4 | |||
| TC1 | 108/38,663 | 0.28 | 1.0 |
| ArtN/N (82.10.66.2) | 585/458,000 | 0.13 | 0.5 |
| DNA-PKcsN/N | 173/106,000 | 0.16 | 0.6 |
| Experiment 5 | |||
| TC1 | 902/478,500 | 0.19 | 1.0 |
| ArtN/N (82.10.66.2) | 1,128/451,400 | 0.25 | 1.3 |
| DNA-PKcsN/N | 1,559/255,500 | 0.61 | 3.2 |
a
pJH290 (coding joining) for Experiments 1–8.
b
pJH200 (signal joining) for Experiments 1–5.