Figure 3.

Repertoire analysis by single cell DNA-PCR for coamplification of CW3-specific TCR VDJ-β and VJ-α sequences together with DJ-β rearrangements as potential clonal markers. For each of three mice (M-41, M-42, and M-43), single pCW3Kd⁺Vβ10⁺CD8⁺ splenocytes were sorted 2 wk after immunization with P815-CW3 tumor cells. The rearranged TCR-α and TCR-β nucleotide sequences were amplified by single cell DNA-PCR. PCR products from cells with successful amplifications for TCR-α and TCR-β rearrangements were sequenced to identify paired αβ TCRs. Other details are as described for Fig. 2.