Figure 4.

Two clones expressing CW3-specific αβ TCRs encoded by an identical VDJ-β but different VαJα sequences share an identical Dβ1-Jβ1.4 rearrangement. Individual cells (identified by #) from M-42 or M-43 were amplified by DNA-PCR as described in Fig. 3 to identify their CW3-specific αβ TCRs and their potential DJ-β rearrangements. Shown for each cell are the codes for the TCR-α and TCR-β sequences, the amplified DJ rearrangements together with a code to indicate D and J gene segment usage, and the unrearranged D2-J2 GL sequences. Where indicated, there was no detectable PCR amplification (na) using the indicated primer combinations. No Dβ1 to Jβ2 rearrangements were found for any of these cells. Sequences in italics shown above each series represent the 3′ or 5′ ends of the relevant genomic D or J sequences, respectively. DJ nucleotide sequences are separated to indicate the D, N-nucleotide, and J portions.