Table I.

Efficient Single Cell RT-PCR and DNA-PCR Amplification of CW3-specific αβ TCRs from Individual Mice ^a

	M-2RT-PCR		M-3RT-PCR		M-33RT-PCR		M-41DNA-PCR		M-42DNA-PCR		M-43DNA-PCR
	n	%	n	%	n	%	n	%	n	%	n	%
Amplification:b
TCR-β+ cells	91	63.2	114	79.2	115	79.9	70	54.7	62	48.4	79	41.1
TCR-α1 cells	76	52.8	91	63.2	58	40.3	85	66.4	70	54.7	135	70.3
Total cells amplified	144		144		144		128		128		192
Cells with seq's for:c
CW3-TCR-β	78	54.2	114	79.2	115	79.9	nae	na	na	na	na	na
CW3-TCR-α	71	49.3	90	62.5	57	39.6	na	na	na	na	na	na
CW3-αβ TCR	48	33.3	79	54.9	45	31.2	46	35.9	37	28.9	57	29.7
No. of different CW3-TCRs:d
αβ TCRs	15		18		10		15		11		16
Additional TCR-β's	1		3		5		na		na		na
Additional TCR-α's	3		0		0		na		na		na

^a2 wk after immunization with P815-CW3 transfectants, Vβ10⁺CD8 T cells gated as CD62L⁻ (M-2 and M-3) or pCW3Kd⁺ (M-33, M-41, M-42, and M-43) were sorted as single cells and TCR sequences were amplified by RT-PCR or DNA-PCR.

^bThe number and percent of cells for which a TCR-β or TCR-α sequence was amplified.

^cThe number and percent of cells for which a CW3-like TCR-β, TCR-α, or paired αβ TCR was amplified.

^dThe number of different CW3-like αβ TCRs and the number of additional CW3-like TCR-β or TCR-α sequences found without a partner.

^eNot applicable because we only determined sequences for cells from which both a TCR-α and TCR-β PCR product was detected.