Figure 2.

Apoptosis-associated mitochondrial cytochrome c releases in Jurkat but not neutrophils. (A) Jurkat cells were induced to undergo apoptosis by treated with 500 nM staurosporine or 20 μM actinomycin D and mitochondrial and cytosolic fractions were prepared at the indicated times as described in Materials and Methods. Cell fractions were probed with the indicated antibodies. (B) Primary human neutrophils and Jurkat cells were treated with 500 nM staurosporine or 20 μM actinomycin D followed by preparation of mitochondrial and cytosolic fractions. Cell fractions were then probed for cytochrome c and MnSOD as indicated.