Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 Feb 3;197(3):303–314. doi: 10.1084/jem.20020717

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — Expression of Fas and FasL in control and IRF-4 transfectants. (A) Surface expression of the Fas receptor in Jurkat transfectants. Cells from control and IRF-4–transfected cells were stained with either a PE-labeled isotype-matched control Ab (I) or a PE-labeled anti-Fas IgG1 antibody (II). Cells were subsequently analyzed by flow cytometry. Control vector transfectants (solid lines); IRF-4 transfectants (dotted lines). (B) RT-PCR analysis for FasL expression in IRF-4 Jurkat transfectants. Total RNA was isolated from control and IRF-4–transfected cells, and RT-PCR was performed using primers specific for FasL (top) or GAPDH (bottom). PCR products were separated by electrophoresis on a 2% agarose gel.

Figure 2. — Expression of Fas and FasL in control and IRF-4 transfectants. (A) Surface expression of the Fas receptor in Jurkat transfectants. Cells from control and IRF-4–transfected cells were stained with either a PE-labeled isotype-matched control Ab (I) or a PE-labeled anti-Fas IgG1 antibody (II). Cells were subsequently analyzed by flow cytometry. Control vector transfectants (solid lines); IRF-4 transfectants (dotted lines). (B) RT-PCR analysis for FasL expression in IRF-4 Jurkat transfectants. Total RNA was isolated from control and IRF-4–transfected cells, and RT-PCR was performed using primers specific for FasL (top) or GAPDH (bottom). PCR products were separated by electrophoresis on a 2% agarose gel.