Figure 1.
TH3Z hybridoma recognizes a polymorphic H antigen which is highly expressed in BMDCs and is presented by the Ab MHC II molecule. (A) The indicated number of cells derived from C57BL/6 (B6) or MHC-matched 129/J mice were cocultured with the lacZ-inducible TH3Z hybridoma. (B) 105/well B6 spleen cells were cultured with TH3Z T cells in the presence of the indicated mAb or medium alone. (C) Bone marrow cells were cultured for the indicated days and varying number of cells were used as APCs for stimulating TH3Z T cells. The lacZ response of the TH3Z hybridoma after an overnight incubation was determined after lysis of the cultures and addition of the lacZ substrate CPRG. The absorbance of the cleaved chlorophenol red product at 595 nm is shown for each culture.