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. 2003 Feb 3;197(3):375–385. doi: 10.1084/jem.20021961

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Copyright © 2003, The Rockefeller University Press

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Figure 6. — Non-self at the H46 locus is defined by a single amino acid polymorphism. (A) The deduced amino acid sequences of H46 cDNA from the B6 (H46a), the congenic 21M (H46 ^b), and the parental 129/J (H46 ^b) mouse strains. The cDNA fragments corresponding to the B6-derived clone 224–26 were obtained by RT-PCR. The H46^a antigenic peptide is boxed. (B) Constructs containing the H46^a and H46^b wild-type as well as single point mutations of each of the two polymorphic residues H46^a P>L and H46^a A>M subcloned into the bacterial expression vector pLEX. (C) TH3Z lacZ response to 129/J BMDCs fed with bacteria expressing the constructs shown in B. (D) Amino acid sequences of the synthetic peptide representing the H46^a and H46^b homologues. Bold letters indicate the single amino acid polymorphism between the B6 and 129 strains. (E) TH3Z lacZ response to the H46^a and H46^b peptides presented by 129/J-derived BMDCs. (F) The relative A^b MHC II binding property of H46^a and H46^b peptides compared with the A^k MHC II binding peptides HEL34–45 and OVA247–265. The data show the varying concentrations of indicated peptides cause competitive inhibition of the binding of the Eα peptide to the A^b MHC II on A^b-L cells. The Eα/A^b complex was detected with the Yae mAb by flow cytometry as described in Materials and Methods.