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. 2003 Feb 3;197(3):375–385. doi: 10.1084/jem.20021961

Figure 7.

Figure 7.

The H46a/Ab complex is generated from the full-length IL4i1 gene via an endogenous presentation pathway. (A) 129/J BMDCs were transduced with retroviruses expressing either vector alone or the B6 or 129/J-derived full-length IL4i1 cDNAs as described in Materials and Methods. 2 d later the transduced cells were titrated and used as APC for TH3Z T cells. About 8–10% cells expressed the retrovirus as indicated by the presence of CD11c+GFP+ cells. (B) COS cells were transiently transfected with the indicated cDNA constructs representing the Ab MHC II subunits Ab α and Ab β together with the B6 or 129/J derived full-length IL4i1 genes or the 224–26 fragment. After 48 h, indicated number of transfected cells were cocultured with TH3Z T cells and their lacZ response was measured after overnight incubation. (C and D) The H46a/Ab epitope is not acquired by BMDC from other cells expressing the precursor gene. COS cells were transfected with the B6-derived full-length IL4i1 cDNA with or without the Ab subunits. 2 d later indicated numbers of transfected COS cells (C) or BMDCs from B6 (H46 a+, Ab+) or B10.D2 (H46 a+, Ab-). (D) were added to 129/J BMDC (H46 b, Ab+). The lacZ response of TH3Z T cells was obtained only when the H46 a precursor and the Ab MHC II is expressed in the same cells.