Table I.
Oligonucleotide Primers
| Construct | Forward (5′–3′) | Reverse (5′–3′) | Figure |
|---|---|---|---|
| ΔN1 | CCGGAATTCCATCACGTGTTCGTGGAGACT | TGTAAAACGACGGCCAGTGC | 5, A and B |
| ΔN2 | CCGGAATTCCATGAGTGGCATGGTCATGGG | TGTAAAACGACGGCCAGTGC | 5, A and B |
| ΔN3 | CCGGAATTCCATTCGCAAAGAGGAAGTGAG | TGTAAAACGACGGCCAGTGC | 5, A and B |
| ΔC1 | GGTGACGCTCTTAAAAATTAAGCC | GACTCTAGACGTTTCCACCAACAAGTG | 5, A and B |
| ΔC2 | GGTGACGCTCTTAAAAATTAAGCC | GACTCTAGAATATATATTCTGGTGGGC | 5, A and B |
| ΔC3 | GGTGACGCTCTTAAAAATTAAGCC | GACTCTAGATTGCGATTAGGAGTGGTC | 5, A and B |
| H46b | CCGGAATTCCATGTCAGCCCAGATGAACAG | GACTCTAGATTGCGATTAGGAGTGGTC | 6, A–C |
| H46b
P>L |
CAGATGAACAGGAGCTCTCTCACAAACAC | GTGTTTGTGAGAGAGCTCCTGTTCATCTG | 6, B and C |
| H46b
A>M |
GAGGGGCAGCAACACATGTTTGTGGAGGCCA | TGGCCTCCACAAACATGTGTTGCTGCCCCTC | 6, B and C |
| IL4i1 | CCGGAATTCATGGCTGGGCTGGCCCTG | ATAGTTTAGCGGCCGCTTGCGATTAGGAGTGGT | 7, B, C, and D |