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. 2003 Feb 3;197(3):297–302. doi: 10.1084/jem.20021343

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Copyright © 2003, The Rockefeller University Press

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Figure 3. — BLyS processing takes place at the intracellular level. (A) 1 ng/lane rBLyS and whole-cell extracts prepared from 40 μg unstimulated HL-60 cells, and from 150 μg neutrophils incubated for 21 h with or without 1,000 U/ml G-CSF, in the absence or presence of 25 μM CMK, were electrophoresed, blotted, and analyzed for BLyS protein expression using a specific anti-BLyS polyclonal Abs. The top arrow indicates the nonprocessed 32-kD BLyS, whereas the bottom arrow indicates the cleaved 17-kD BLyS. The experiment shown is representative of three experiments. (B) Neutrophils (5 × 10⁶/ml) were cultured for 21 h with 1,000 U/ml G-CSF, in the absence or presence of 25 μM CMK. Culture supernatants were harvested and examined for BLyS and IL-1ra content. Values represent means ± SEM of duplicate determinations calculated from three independent experiments.

Figure 3. — BLyS processing takes place at the intracellular level. (A) 1 ng/lane rBLyS and whole-cell extracts prepared from 40 μg unstimulated HL-60 cells, and from 150 μg neutrophils incubated for 21 h with or without 1,000 U/ml G-CSF, in the absence or presence of 25 μM CMK, were electrophoresed, blotted, and analyzed for BLyS protein expression using a specific anti-BLyS polyclonal Abs. The top arrow indicates the nonprocessed 32-kD BLyS, whereas the bottom arrow indicates the cleaved 17-kD BLyS. The experiment shown is representative of three experiments. (B) Neutrophils (5 × 10⁶/ml) were cultured for 21 h with 1,000 U/ml G-CSF, in the absence or presence of 25 μM CMK. Culture supernatants were harvested and examined for BLyS and IL-1ra content. Values represent means ± SEM of duplicate determinations calculated from three independent experiments.

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