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. 2003 Mar 17;197(6):735–742. doi: 10.1084/jem.20021153

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Copyright © 2003, The Rockefeller University Press

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Figure 5. — Effect of an intranasal treatment with FHA on colonization by BPLC6. Balb/c mice were treated by an intranasal instillation of either 20 μl of PBS containing 0.5 M NaCl (PBS/NaCl) and 5 μg of purified FHA or 20 μl of PBS/NaCl, and then immediately infected intranasally with ∼5 × 10⁴ CFU of B. pertussis BPLC6 (SphB1⁻). At the indicated time points, mice were killed, and the viable bacteria present in the lungs were counted. Black bars, numbers of BPLC6 after mock treatment, white bars, numbers of BPLC6 after the FHA treatment. *P < 0.05 relative to the numbers of BPLC6 CFU administered without FHA.