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RNA-FISH confirms biallelic κ germline transcription. A Cy3 labeled probe (pSPIg8) for the κ germline transcript revealed biallelic transcription in 91% of the RAG-1−/− Abelson-transformed pre-B cells in which any κ germline transcript was detected. DAPI allows visualization of DNA in nuclei. Two of the four cells in the field presented are expressing the κ germline transcript and both of them have two RNA-FISH signals.
