Table I.
Analysis of β2 Microglobulin Allelic Expression in a Mixture of Spretus and Musculus B Lymphocytes
| Positive PCRs per cell |
No. of cells | Percent monoallelic |
|
|---|---|---|---|
| 8 | 2 | 100% | 2M |
| 7 | 1 | 100% | 1M |
| 6 | 1 | 100% | 1S |
| 5 | 5 | 100% | 2M, 3S |
| 4 | 2 | 100% | 2M |
| 3 | 5 | 100% | 3M, 2S |
| 2 | 1 | 100% | 1M |
| 1 | 5 | 100% | 4M, 1S |
| 0 | 6 |
The analysis was performed on 28 cells. M, musculus; S, spretus. As expected, this control reconstruction experiment shows that when we artificially create pure “monoallelic” expression, the single cell RTPCR assay can indicate monoallelic expression consistently.