Figure 6.

LPS-treated CD4⁺ CD25⁺ are efficient regulatory cells in vivo. RAG-1–deficient animals (three per experimental group) received 10⁵ CD4⁺ CD45RB^high Thy1.2 cells alone or together with the same number of CD4⁺ CD25⁺ Thy1.1 cells, either freshly isolated or maintained in culture sequentially with LPS and IL-2 for a total of 6 d (as described in Fig. 4). Mice were analyzed at 21 d after transfer. (a) Control of wasting disease. The white dot represents an animal found dead on day 17 after transfer. Its weight monitored on day 16 was used. (b) Control of lymphocyte expansion. Numbers of CD4⁺ Thy1.2⁺ cells (originally CD45RB^high) recovered in the mesenteric LNs. (c) Viability and phenotype of the transferred CD4⁺ CD25⁺ cells. Representative staining of axillary LNs from recipients of cotransfers.