Table I.
Primer Sequences for Mouse TLR RT-PCR Used in This Study
| Nucleic sequencesa | Tpb | |
|---|---|---|
| TLR-1 | TCTCTGAAGGCTTTGTCGATACA | 56 |
| GACAGAGCCTGTAAGCATATTCG | ||
| TLR-2 | TCTAAAGTCGATCCGCGACAT | 58 |
| TACCCAGCTCGCTCACTACGT | ||
| TLR-3 | TTGTCTTCTGCACGAACCTG | 58 |
| CGCAACGCAAGGATTTTATT | ||
| TLR-4 | CAAGAACATAGATCTGAGCTTCAACCC | 62 |
| GCTGTCCAATAGGGAAGCTTTCTAGAG | ||
| TLR-5 | ACTGAATTCCTTAAGCGACGTA | 56 |
| AGAAGATAAAGCCGTGCGAAA | ||
| TLR-6 | AACAGGATACGGAGCCTTGA | 58 |
| CCAGGAAAGTCAGCTTCGTC | ||
| TLR-7 | TTCCGATACGATGAATATGCACG | 56 |
| TGAGTTTGTCCAGAAGCCGTAAT | ||
| TLR-8 | GGCACAACTCCCTTGTGATT | 58 |
| CATTTGGGTGCTGTTGTTTG | ||
| TLR-9c | CCGCAAGACTCTATTTGTGCTGG | 62 |
| TGTCCCTAGTCAGGGCTGTACTCAG |
a
Sequences are written 5′ to 3′, sense and antisense primers successively.
b
Annealing temperature in °C.
c
Reference 48.