Figure 7.
B cells isolated from IL-10 KO/H2q mice fail to differentiate into a “regulatory” phenotype. Spleens were isolated from arthritic IL-10 KO/H2q mice or from the control IL-10 wild-type mice. Splenocytes were cultured for 48 h with CII/isotype control, CII/anti-CD40. B cells were enriched by negative selection, using an anti-CD43 magnetic beads–conjugated antibody, and 5 × 105 cells were transferred intraperitoneally, at the time of CII/CFA immunization, to IL-10 wild-type mice. Arthritis evolution and severity was measured as described previously. Data shows incidence of arthritis (a) and mean of clinical score ± SE (b), and are representative of three experiments. Groups of mice were compared by statistical analysis using the Fisher exact test.