Figure 1.

Subcutaneous challenge of MC57-SIY, but not MC57-L^d, induced proliferation and activation of 2C T cells. MC57G tumor cells were transfected with pcDNA-EGFP (A), pcDNA-L^d (B), or pcDNA-SIY-EGFP (C). MC57-L^d and MC57-SIY expressed L^d and EGFP, respectively (D). 3 × 10⁶ CFSE-labeled 2C T cells were transferred into B6 mice. 24 h later, these mice were challenged with 10⁶ MC57-SIY, MC57-L^d, or MC57-EGFP above the tail base. MC57-SIY–induced proliferation (G), up-regulation of CD44 (H), and secretion of INF-γ (I) of 2C T cells 96 h after tumor challenge in the DLN, NDLN, and spleen. However, MC57-EGFP (E) and MC57-L^d (F) failed to cause any proliferation, up-regulation of CD44 (H), and production of INF-γ (I).