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. 2003 Apr 21;197(8):1017–1028. doi: 10.1084/jem.20021047

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Copyright © 2003, The Rockefeller University Press

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Figure 6. — MMP-9 is expressed by Mac-1⁺ cells not CD4⁺ T cells. (A) RT-PCR analysis of cell populations isolated from an HPV16 tumor by FACS^®. Total RNA from CD4⁺, Mac-1⁺/Gr-1⁺, and unlabelled cells was made and reverse transcribed into cDNA. PCR for MMP-9 (35 cycles), CD4 (35 cycles), and L19 (housekeeping gene; 31 cycles) was performed on the cDNA from the three populations. The product was run on a 1.5% agarose gel and the results are shown with +RT and –RT displayed for each population of cells. (B) Immunolocalization of MMP-9 (red) and Mac-1 (green) in a tumor from an HPV16 mouse. MMP-9 and Mac-1 are found to be colocalizing (yellow) on many cells within the tumor. The inset shows a higher magnification of several cells staining for both MMP-9 and Mac-1. Bar: 20 μm; 10 μm (inset). (C) Immunolocalization of MMP-9 (red) and CD4 (green) in a tumor from an HPV16 mouse. No colocalization of MMP-9 and CD4 was observed. Bar: 7 μm.