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. 2003 Apr 21;197(8):1029–1035. doi: 10.1084/jem.20021321

Figure 4.

Figure 4.

Overexpression of JDP2 stimulates TRAP and cathepsin K gene promoters in RAW264.7 cells. (A) RAW264.7 cells were cotransfected with indicated expression vector for JDP2 or RANK, together with the firefly luciferase reporter construct (pGL3-TRAP or pGL3-CTSK) and pRL-SV40. Cells were either untreated or treated for 24 h with 100 ng/ml sRANKL before being collected. Relative luciferase activity was determined, and the level of induction is indicated as a ratio with respect to cells transfected with the empty vector in the absence of sRANKL. Values shown are means ± SD of a representative of at least two independent experiments performed in triplicate. (B) RAW264.7 cells were cotransfected with the indicated dose of expression vectors for JDP2 with a COOH-terminal HA epitope (JDP2-HA), together with the firefly luciferase reporter construct (pGL3-TRAP or pGL3-CTSK) and pRL-SV40. The total amount of DNA in each transfection was kept constant by adding empty vector (pCR3.1) DNA. Cells were either untreated or treated for 24 h with 100 ng/ml sRANKL before being collected. Relative luciferase activity was determined as described in A. Values shown are means ± SD of a representative experiment performed in triplicate.