TABLE 2.
High-salinity-repressed genes
| Genea | Functionb | Signal strength ratioc for cells growing in MM with:
|
|
|---|---|---|---|
| 1.2 M NaCl, 5 μM Fe | 1.2 M NaCl, 250 μM Fe | ||
| ybdO | Unknown | 4.6 | 3.3 |
| ybfG | Unknown; similar to unknown proteins | 3.6 | 2.5 |
| ycbJ | Unknown; similar to macrolide 2′-phosphotransferase | 5.6 | 4.5 |
| ycbU | Unknown; similar to NifS protein homolog | 4.9 | 3.2 |
| ldh | l-Lactate dehydrogenase | 26.0 | 0.6 |
| lctP | l-Lactate permease | 4.2 | 0.7 |
| ydaD | Unknown; similar to alcohol dehydrogenase | 3.5 | 2.0 |
| ydcR | Unknown; similar to transposon protein | 5.1 | 1.5 |
| ydfR | Unknown; similar to unknown proteins | 3.2 | 2.1 |
| yfmT | Unknown; similar to benzaldehyde dehydrogenase | 8.6 | 4.5 |
| yfmS | Unknown; similar to methyl-accepting chemotaxis protein | 11.4 | 12.7 |
| pel | Pectate lyase | 4.3 | 5.0 |
| yfiQ | Unknown; similar to surface adhesion protein | 3.4 | 2.1 |
| katA | Vegetative catalase 1 | 4.4 | 21.3 |
| glpD | Glycerol-3-phosphate dehydrogenase | 3.0 | 3.2 |
| lytF | Gamma-d-glutamate-meso-diaminopimelate muropeptidase (major autolysin) | 11.3 | 6.5 |
| yhdO | Unknown; similar to 1-acylglycerol-3-phosphate O-acyltransferase | 3.8 | 3.4 |
| yhxC | Unknown; similar to alcohol dehydrogenase | 3.3 | 1.7 |
| yjfB | Unknown | 3.0 | 3.1 |
| motB | Motility protein (flagellar motor rotation) | 22.3 | 9.6 |
| motA | Motility protein (flagellar motor rotation) | 26.4 | 9.7 |
| mcpC | Methyl-accepting chemotaxis protein | 4.1 | 4.2 |
| abh | Transcriptional regulator of transition state genes (AbrB-like) | 11.5 | 16.8 |
| ylqB | Unknown | 10.0 | 4.0 |
| flgB | Flagellar basal-body rod protein | 3.6 | 3.3 |
| flgC | Flagellar basal-body rod protein | 3.2 | 2.7 |
| fliF | Flagellar basal-body M-ring protein | 5.9 | 4.5 |
| fliG | Flagellar motor switch protein | 6.4 | 5.3 |
| fliH | Flagellar assembly protein | 5.9 | 4.6 |
| fliI | Flagellum-specific ATP synthase | 4.7 | 3.8 |
| fliJ | Flagellar protein required for formation of basal body | 5.1 | 4.5 |
| ylxF | Unknown; similar to unknown proteins | 25.7 | 15.5 |
| ylxG | Unknown; similar to flagellar hook assembly protein | 6.8 | 7.1 |
| flgE | Flagellar hook protein | 7.1 | 7.5 |
| fliL | Flagellar protein required for flagellar formation | 7.3 | 6.7 |
| fliM | Flagellar motor switch protein | 9.0 | 10.4 |
| fliY | Flagellar motor switch protein | 5.4 | 4.0 |
| cheY | Two-component response regulator involved in modulation of flagellar switch bias | 4.5 | 4.2 |
| fliZ | Flagellar protein required for flagellar formation | 6.1 | 5.7 |
| fliP | Flagellar protein required for flagellar formation | 9.3 | 9.9 |
| fliR | Flagellar protein required for flagellar formation | 3.4 | 4.4 |
| flhB | Flagellum-associated protein | 5.9 | 7.5 |
| flhA | Flagellum-associated protein | 6.0 | 4.9 |
| flhF | Flagellum-associated protein | 7.6 | 5.2 |
| ylxH | Unknown; similar to flagellar biosynthesis switch protein | 5.2 | 5.0 |
| cheC | Inhibition of CheR-mediated methylation of MCPs | 5.6 | 7.2 |
| sigD | RNA polymerase flagella, motility, chemotaxis, and autolysis sigma factor | 3.4 | 4.5 |
| ylxL | Unknown; similar to unknown proteins | 3.3 | 4.2 |
| yoeB | Unknown | 40.9 | 28.9 |
| yolA | Unknown | 7.5 | 6.1 |
| trpF | Phosphoribosyl anthranilate isomerase | 4.8 | 14.5 |
| trpC | Indole-3-glycerol phosphate synthase | 4.3 | 7.7 |
| sigX | RNA polymerase ECF-type sigma factor | 3.7 | 2.9 |
| tasA | Translocation-dependent antimicrobial spore component | 28.9 | 41.9 |
| sipW | Signal peptidase 1 | 5.5 | 5.8 |
| hemC | Porphobilinogen deaminase | 3.1 | 2.1 |
| yteR | Unknown; similar to unknown proteins | 3.2 | 1.9 |
| tlpB | Methyl-accepting chemotaxis protein | 3.4 | 2.6 |
| mcpA | Methyl-accepting chemotaxis protein | 11.3 | 8.9 |
| mcpB | Methyl-accepting chemotaxis protein | 3.6 | 3.0 |
| maeN | Na+/malate symporter | 3.2 | 1.6 |
| guaC | GMP reductase | 3.4 | 3.0 |
| yusP | Unknown; similar to multidrug efflux transporter | 5.2 | 9.9 |
| mrgA | Metalloregulation DNA-binding stress protein | 3.2 | 4.7 |
| yvfE | Unknown; similar to spore coat polysaccharide biosynthesis | 19.1 | 10.6 |
| yvfC | Unknown; similar to UDP-galactose phosphate transferase | 4.7 | 3.4 |
| yveS | Unknown; similar to unknown proteins | 3.5 | 2.5 |
| yveO | Unknown; similar to glycosyltransferase | 3.1 | 3.1 |
| yveK | Unknown; similar to capsular polysaccharide biosynthesis | 4.2 | 3.2 |
| yvzB | Unknown; similar to flagellin | 14.2 | 11.5 |
| yvjB | Unknown; similar to carboxy-terminal processing protease | 3.8 | 3.3 |
| fliT | Flagellar protein | 3.8 | 4.3 |
| fliS | Flagellar protein | 8.2 | 7.1 |
| fliD | Flagellar hook-associated protein 2 (HAP2) | 14.5 | 9.5 |
| hag | Flagellin protein | 21.2 | 10.3 |
| flgK | Flagellar hook-associated protein 1 (HAP1) | 3.1 | 3.5 |
| yvyG | Unknown; similar to flagellar protein | 3.7 | 4.2 |
| ywtD | Unknown; similar to murein hydrolase | 8.8 | 5.0 |
| flhP | Flagellar hook-basal body protein | 4.6 | 4.2 |
| flhO | Flagellar basal-body rod protein | 7.9 | 5.2 |
| thrZ | Threonyl-tRNA synthetase (minor) | 3.6 | 3.6 |
| ywbG | Unknown; similar to unknown proteins | 3.1 | 2.0 |
| epr | Minor extracellular serine protease | 6.9 | 4.8 |
| dltA | d-Alanyl-d-alanine carrier protein ligase | 5.8 | 6.6 |
| dltB | d-Alanine transfer from Dcp to undecaprenol phosphate | 3.8 | 5.8 |
| dltE | Involved in lipoteichoic acid biosynthesis | 5.4 | 5.7 |
| cydD | ABC transporter required for expression of cytochrome bd (ATP-binding protein) | 3.2 | 0.4 |
| cydC | ABC transporter required for expression of cytochrome bd (ATP-binding protein) | 3.1 | 0.6 |
| yxkC | Unknown | 8.4 | 4.7 |
| yxjH | Unknown; similar to unknown proteins from B. subtilis | 3.4 | 3.7 |
| yxjG | Unknown; similar to unknown proteins from B. subtilis | 3.1 | 3.8 |
| deaD | ATP-dependent RNA helicase | 3.7 | 3.2 |
| yxiM | Unknown; similar to rhamnogalacturonan acetylesterase | 3.6 | 3.1 |
| yxzC | Unknown | 5.6 | 4.2 |
| yxxG | Unknown | 9.4 | 9.9 |
| wapA | Cell wall-associated protein precursor | 7.7 | 6.9 |
| hutI | Imidazolone-5-propionate hydrolase | 3.0 | 1.8 |
| iolH | myo-Inositol catabolism | 3.1 | 3.3 |
| idh | myo-Inositol 2-dehydrogenase | 3.5 | 2.3 |
| yxaL | Unknown; similar to serine/threonine protein kinase | 3.3 | 0.9 |
| yxaD | Unknown; similar to transcriptional regulator (MarR family) | 4.4 | 4.3 |
a
Sorted according to the order in the B. subtilis genome. Members of the DegS/DegU regulon are boldfaced.
b
Functions are derived from the SubtiList database.
a
Ratio between the signal strength in cells propagated under the conditions indicated and the signal strength in cells continuously growing in synthetic medium with 5 μM FeCl3 and no extra NaCl added.