Table I.
Reactivity of Rat CD28-specific mAb with Mouse and Rat CD28 as well as Different Chimeric and Mutant CD28 Molecules
| Antibody | MouseCD28 | RatCD28 | m CD28S62P, A64V,E65G | m/r CD281-66 | m CD28 S62P, A64V,E65G, D71N, R109K | m CD28 S62P, A64V,E65G, D71N, R109K, F98V |
|---|---|---|---|---|---|---|
| control | − | − | − | − | − | − |
| 37.51 | +++ | − | +++ | − | +++ | − |
| JJ316 | − | +++ | +++ | − | +++ | +++ |
| JJ319 | − | +++ | − | +++ | − | +++ |
| 5S28 | − | ++ | − | ++ | − | ++ |
| 5S38.17 | − | ++ | ++ | − | ++ | ++ |
| 5S247 | − | +++ | − | +++ | − | +++ |
| 5G40/2 | − | +++ | − | +++ | − | +++ |
| 5G87 | − | ++ | − | ++ | − | ++ |
| 5G111 | − | ++ | − | ++ | − | ++ |
| 5S35 | − | +++ | + | +++ | − | +++ |
+++, very strong reactivity in FACS® analysis; ++, strong reactivity; +, weak reactivity; −, no reactivity. As controls, an isotype-matched irrelevant mAb and the mouse CD28-specific 37.51 mAb were included.