FIG. 1.

Physical and restriction map of the P. denitrificans chromosomal DNA region containing the pazS gene. The map at the top is a restriction map. B, BamHI; Bc, BclI; E, EcoRI; M, MunI; Sm, SmaI; Sp, SphI; X, XhoI. The arrows indicate the positions of the pazS and nosC genes and the nosR 5′ region. The lines below the map are the P. denitrificans DNA fragments cloned in this study. Ω and Km^r indicate the positions of Ω (spectinomycin-streptomycin) and kanamycin resistance cassettes, respectively.