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Detection of cytochrome cd1 in total soluble extracts from wild-type and mutant strains of P. denitrificans. Proteins were separated by SDS-PAGE, and cytochrome cd1 was detected by immunoblotting. Lane 1, molecular weight markers; lane 2, Pd1222 (wild type); lane 3, Pd21.31 (cycA); lane 4, IP1013 (pazS::Kmr); lane 5, IP1121 (cycA pazS::Kmr). The lanes contained approximately 20 μg of total protein.
