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. 2003 Apr 7;197(7):907–918. doi: 10.1084/jem.20021366

Figure 8.

Figure 8.

DCs can mediate the negative selection of NKT cells. 107 bone marrow–derived cells from WT mice and 3.3 × 106 DCs (CD11c+) from either WT or CD1dTg mice were cotransferred to irradiated RAG-deficient mice (700 rad) by intravenous injection. After 10 wk, spleen and liver lymphocytes from chimera were isolated and analyzed by flow cytometry. (a) Flow cytometry analysis of CD1d expression on bone marrow-derived DCs (CD11c+, greater than 80% are CD11b+CD8−) from donor mice and thymic DCs (CD11c+, ∼50–60% CD11b+CD8−, ∼30–40% CD11b−CD8+) from reconstituted mice. The levels of CD1d expression on bone marrow–derived DCs from Tg animals is ∼10–20 fold higher than those of WT controls. Specific fluorescence profiles (thin line: WT DCs and thick line: Tg DCs) obtained with anti-CD1d (5C6) were overlayed onto background profiles (dotted line) obtained with isotype control Ab. (b) Absolute NKT cell numbers of liver lymphocytes and splenocytes from each group of chimeras. The absolute number of NKT cells was calculated by percentage of NKT cells × total number of cells. Data shown represent mean ± SE of three mice in each group. (c) Cytokine production by NKT cells of liver lymphocytes and splenocytes from each group of chimeras was determined as described in Fig. 7.