Figure 5.

Plaque assays of cell-free culture supernatants show infectivity. Supernatants transmit DV infection from DV-infected DCs (left) and THP DC-SIGN cells (right) to Vero cells. DCs or THPs were pretreated with an anti–DC-SIGN mAb (clone 120612) or a matched isotype control and exposed to DV for 2 h. Virus and mAb were washed away, and the supernatants were collected 48 h later. Plaque assays were used to determine PFUs in the collected supernatants. Data are the averages of two independent experiments.