Table II.

Number (×10³) of Type 1 Cytokine-producing CD4⁺ and CD8⁺ T Cells in Lung after B. dermatitidis Infection as Measured by Intracellular Cytokine Staining

	IFN-γ		TNF-α		GM-CSF
Day	CD4+	CD8+	CD4+	CD8+	CD4+	CD8+
Unvaccinated
0	5 ± 2	4 ± 2	13 ± 8	17 ± 9	4 ± 2	0 ± 0
2	1 ± 0	2 ± 1	2 ± 1	5 ± 2	2 ± 1	2 ± 1
4	14 ± 4	13 ± 4	70 ± 33	79 ± 31	20 ± 8	3 ± 1
6	94 ± 44	183 ± 118	263 ± 147	383 ± 214	127 ± 57	40 ± 16
8	186 ± 99	299 ± 166	508 ± 342	879 ± 64	172 ± 91	33 ± 16
12	279 ± 105	424 ± 161	1,438 ± 578	2,108 ± 857	212 ± 83	246 ± 82
Vaccinated
0	23 ± 14	10 ± 6	64 ± 48	40 ± 30	31 ± 20	1 ± 1
2a	92 ± 88	36 ± 30	178 ± 197	87 ± 88	87 ± 80	16 ± 13
4b	325 ± 257	226 ± 192	1,355 ± 981	954 ± 743	593 ± 426	187 ± 164
6	294 ± 126	111 ± 61	1,821 ± 855	498 ± 301	508 ± 265	24 ± 14
8	315 ± 121	60 ± 33	2,510 ± 953	291 ± 157	920 ± 350	13 ± 7
12	225 ± 105	64 ± 38	1,890 ± 868	388 ± 196	605 ± 331	25 ± 13
Vaccinated/CD4− c
0		3 ± 1		7 ± 4		2 ± 1
2 a		22 ± 12		102 ± 97		21 ± 12
4 b		501 ± 141		1,779 ± 86		312 ± 107
6		334 ± 133		1,009 ± 673		248 ± 104
8		206 ± 62		1,015 ± 422		172 ± 53
12		201 ± 119		1,234 ± 758		263 ± 162

Data represent an average of four independent experiments (lungs of 6–12 mice per group were pooled at each time point). Mice were infected with 2 × 10³ yeast and analyzed serially after infection.

^aP ≤ 0.01 vs. unvaccinated mice for all three cytokines.

^bP ≤ 0.005 vs. unvaccinated mice for all three cytokines.

^cC57BL/6 mice were depleted of CD4⁺ T cells during the induction and expression phase of vaccine immunity.