Table II.

Comparison of Two Independent PKCθ Knockout Strains

	Sun et al. (reference 14)	This study
Targeting strategy:	Exon 11 replaced by PKG-neo	Cre/loxP null allele (deletion of exons 3 and 4)
Residual open reading frame	365 aa	9 aa
Thymocytes:
Development	normal	normal
Peripheral T cells:
Proliferative responses
to CD3/CD28	impaired	impaired
to PMA/ionomycin	impaired	normal
to allogenic MHC	partially impaired	partially impaired
IL-2 production
to CD3/CD28	impaired	impaired
to PMA/ionomycin	impaired	normal
Heterozygous phenotype:	impaired	normal
EMSA/stimulated with CD3/CD28
NF-κB	impaired	partially impaired
AP-1	impaired	partially impaired
NFAT	normal	impaired
Surface expression/stimulated with CD3/CD28
CD69	impaired	normal
CD25	impaired	normal