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. 2002 Aug 5;196(3):407–412. doi: 10.1084/jem.20020470

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Copyright © 2002, The Rockefeller University Press

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Figure 3. — The mAb 2A11 recognizes Dectin-1 and detects its expression on the surface of primary macrophages. (A) The mAb 2A11, but not an IgG2b control mAb, recognizes Dectin-1, and can specifically immunoprecipitate soluble recombinant hemagglutinin (HA)-tagged Dectin-1. (B) The mAb 2A11 stains Dectin-1 transductants (shaded histogram), but not nontransduced cells (unshaded histogram), by flow cytometry. (C) 2A11 detects Dectin-1 expression on the surface of freshly isolated F4/80⁺CR3⁺ Tg macrophages. The gate shown in the left panel denotes the population examined in the right panels. (D) Dectin-1 is expressed at a similar level on the surface of both wild-type and CD11b^−/− bone marrow–derived macrophages.